Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| gene (Mus Musculus) | Bak1 | N/A | ID: 12018 | N/A |
| gene (Mus Musculus) | Bax | N/A | ID: 12028 | N/A |
| cell line (Mus Musculus) | Wild Type SV40 Mouse Embryonic Fibroblasts; WT MEFs | PMID: 11326099 | N/A | N/A |
| cell line (Mus Musculus) | Bax Bak1 double knockout SV40Mouse Embryonic Fibroblasts; DKO MEFs | PMID: 11326099 | N/A | N/A |
| cell line (Mus Musculus) | DKO MEFs stably reconstitutied with wild type Bax; DKO-WT-Bax | PMID: 21255726 | N/A | N/A |
| cell line (Mus Musculus) | DKO MEFs stably reconstitutied with 92-94A mutant Bax; DKO-92-94A-Bax | PMID: 21255726 | N/A | N/A |
| cell line (Mus Musculus) | DKO MEFs stably reconstitutied with 63-65A mutant Bax; DKO-63-65A-Bax | PMID: 21255726 | N/A | N/A |
| antibody | Bax6A7 | Thermo Fisher Scinentific | MA5-14003 | 1 to 100 |
| antibody | TOM20 | Santa Cruz Biotechnology | sc-11415 | 1 to 100 |
| antibody | Lamp-1 | DSHB-University of Iowa | 1D4B | 1 to 200 and 1 to 1000 |
| antibody | Bax | Santa Cruz Biotechnologies | sc-493 | 1 to 300 |
| antibody | Bak | Millipore | 06–536 | 1 to 1000 |
| antibody | LC3I/II | Abcam | ab58610 | 1 to 500 |
| antibody | Bcl-2 | Santa Cruz Biotechnologies | sc-7382 | 1 to 300 |
| antibody | Lamp1 | Abcam | ab24170 | 1 to 500 |
| antibody | Cathepsin D | Novus | AF1029 | 1 to 800 |
| antibody | GAPDH | Fitzgerald | 10R-G109A | 1 to 25,000 |
| antibody | Total OXPHOS Rodent WB Antibody Cocktail | Abcam | ab110413 | 1 to 10,000 |
| antibody | αTubulin | Santa Cruz Biotechnologies | sc-8035 | 1 to 250 |
| recombinant DNA reagent | Lysosomal targeted Bax; Lyso-Bax | Bio Basic | N/A | special order |
| recombinant DNA reagent | MiniSOG-Bax | Bio Basic | N/A | special order |
| sequence-based reagent | N/A | N/A | N/A | N/A |
| peptide, recombinant protein | N/A | N/A | N/A | N/A |
| commercial assay or kit | Lysosome Isolation Kit | Sigma | LYSISO1 Sigma | N/A |
| commercial assay or kit | intracellular pH detection kit | Invitrogen | P35379 | N/A |
| commercial assay or kit | Muse Count and Viability assay | EMD Millipore | MCH100102 | N/A |
| chemical compound, drug | ABT-737 | Selleck Chemicals | S1002 | 20 μM |
| chemical compound, drug | Gossypol | Tocris | 1964 | 10 μM |
| chemical compound, drug | caspase inhibitor zVAD-fmk | Promega | G7232 | 20 μM |
| chemical compound, drug | (S)-O-methyl-serine dodecylamide hydrochloride; MSDH | Avanti Polar Lipids | 850546 | 30 μM |
| chemical compound, drug | staurosporine | Sigma-Aldrich | S6942 | 200 nM |
| chemical compound, drug | LysoTracker Red DND-99 | ThermoFisher Scientific | L7528 | 50 nM |
| chemical compound, drug | Rhodamine-123 | ThermoFisher Scientific | R-302 | 25 μM |
| chemical compound, drug | pHrodo Red AM Intracellular pH Indicator | ThermoFisher Scientific | P35372 | N/A |
| chemical compound, drug | Protease inhibitor cocktail | ThermoFisher Scientific | 88666 | N/A |
| software, algorithm | Excel 2013 | Microsoft Office | N/A | N/A |
| software, algorithm | Power Point 2013 | Microsoft Office | N/A | N/A |