Fig. 7.

Michalis-Menten inhibitor kinetics of rTAO, in presence of increasing concentration of inhibitor CCB. Data plotted is the mean of 3 independent experiments each with triplicate points for each condition. The error plotted is the SEM of the 3 independent experiments. The relative background turnover of substrate without enzyme was subtracted from each condition internally generated within each experiment in triplicate. 2 nM of purified rTAO was used. Graph generated in GraphPad Prism^®.