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. Author manuscript; available in PMC: 2017 Nov 21.
Published in final edited form as: ACS Nano. 2017 Oct 12;11(10):10135–10146. doi: 10.1021/acsnano.7b04717

Figure 3.

Figure 3

In vitro cytolytic activity of OT-1 splenocytes (effector cells) and apoptosis of B16F10-OVA cells (target cells). (a) Deep red fluorescence of B16F10-OVA cell lysates. (b) Region of interest (ROI) fluorescence intensity of B16F10-OVA cell lysates. n = 3 replicates in a representative experiment, mean ± s.d. ****: p < 0.0001 vs. PBS group by Dunnett’s multiple comparisons test. (c) Representative dot plots showing B16F10 cells undergoing apoptosis and necrosis, stained with Annexin V-PI. (d) Percentages of cell population undergoing apoptosis and death determined by dual-color analysis. n = 3 replicates in a representative experiment. ***: p < 0.001 and ****: p < 0.0001 vs. PBS in Σ(apoptotic or dead cells) at each effector to target ratio by Dunnett’s multiple comparisons test.