Table 2.
Total antibody positivity rate after different immunization strategies
| Group | No. mice | Vaccine and administration | Anti‐HCV Abs | Days post‐prime | ||||
|---|---|---|---|---|---|---|---|---|
| Prime | 2xboost | 0 | 14 | 28 | 42 | |||
| Serum IgM | 0/5 | 0/5 | 0/5 | 0/5 | ||||
| 0 | 5 | Serum IgG | 0/5 | 0/5 | 0/5 | 0/5 | ||
| Faeces IgA | 0/5 | 0/5 | 0/5 | 0/5 | ||||
| im | Feeding | Serum IgM | 0/5 | 5/5 | 5/5 | 5/5 | ||
| 1 | 5 | HEK293T‐E1E2 | Lettuce E1E2 | Serum IgG | 0/5 | 0/5 | 0/5 | 4/5 |
| Faeces IgA | 0/5 | 4/5 | 4/5 | 4/5 | ||||
| Feeding | Serum IgM | 0/7 | 0/7 | 1/7 | 2/7 | |||
| 2 | 7 | Lettuce‐E1E2∆N6 | Serum IgG | 0/7 | 0/7 | 0/7 | 0/7 | |
| Faeces IgA | 0/7 | 3/7 | 3/7 | 3/7 | ||||
| Feeding | Serum IgM | 0/5 | 0/5 | 1/5 | 1/5 | |||
| 3 | 5 | Lettuce‐E1E2 | Serum IgG | 0/5 | 0/5 | 0/5 | 0/5 | |
| Faeces IgA | 0/5 | 0/5 | 0/5 | 0/5 | ||||
| 4 | 5 | im | im | Serum IgM | 0/5 | ND | 5/5 | 5/5 |
| HEK293T‐E1E2 | HEK293T‐E1E2 | Serum IgG | 0/5 | ND | 5/5 | 5/5 | ||
| Faeces IgA | ND | ND | 5/5 | 5/5 | ||||
Im, intramuscular; ND, not determined; bold text, antigen specific reactivity