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. 2017 May 26;15(12):1622–1629. doi: 10.1111/pbi.12744

Figure 2.

Figure 2

Molecular analyses of transgenic cotton lines co‐expressing pGR79EPSPS and pGAT. (a) qRT‐PCR analysis of the transgenic expression of pGR79 EPSPS and pGAT in 6‐week‐old transgenic cotton plants. The cotton Actin7 gene was used as control to normalize expression levels. (b) Western blot analysis of the expression of GR79 EPSPS and GAT in transgenic cotton lines GGCO2 and GGCO5. (c) Enzyme‐linked immunosorbent assay (ELISA) analysis of GR79 EPSPS and GAT protein expression in the GGCO2 and GGCO5 cotton lines. (d) ImmunoStrip genotyping of GGCO2 and GGCO5 cotton. The red arrow indicates pGR79 EPSPS capture lines. The blue arrow indicates the control line (anti‐mouse lg G). Total protein extractions from 6‐week‐old cotton leaves were analysed by immunoblotting using anti‐GR79 EPSPS antibodies.