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. 2017 Aug 16;28(12):3579–3589. doi: 10.1681/ASN.2016111222

Figure 5.

Figure 5.

PLD4 upregulates MAPK signaling via TrkA pathway to mediate fibrogenesis. (A) Overview of the PLD4 interaction network on the basis of the immunoprecipitation/mass spectrometry (IP/MS) analysis (respective WDN scores indicated in parentheses). (B) Diagrammatic representation of the localization of interacting partners of PLD4. Orange circles represent PLD4 and gray circles represent the binding partners of PLD4. Immunofluorescence analysis of the subcellular localization of PLD4 by costaining PLD4 with ER (Calnexin)-, Golgi (Golga1)-, and mitochondria (Tom20)-specific proteins in (C) HeLa cells and (D) HEK293 cells. Scale bars, 20 µm. (E) Coimmunoprecipitation of the top three interacting partners of PLD4 (CLGN, LMAN2, and SEL1L, selected on the basis of the WDN score) using PLD4-overexpressed HEK293T cells. Protein expression of CLGN, LMAN2, SEL1L, TrkA, FRS2α, pERK, and ERK in the kidneys of (F) PLD4+/+ and PLD4−/− mice and (G) siRNA (scr and PLD4)-treated mice injected with FA (n=5/group). ER, endoplasmic reticulum; IP, immunoprecipitation; Mito, mitochondria; scr, scrambled; +/+, PLD4 wild-type; −/−, PLD4 knockout.