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. 2017 Aug 11;28(12):3590–3604. doi: 10.1681/ASN.2017020190

Table 2.

List of primers used for real-time RT-PCR (all genes were murine)

Gene Sequence (Sense) Sequence (Antisense) Probe
Mif 5′-CGTGCCGCTAAAAGTCATGA-3′ 5′-GCAAGCCCGCACAGTACAT-3′ SYBR Green
Col 1A1 5′-TCGCTTCACCTACAGCACCC-3′ 5′-TGACTGTCTTGCCCCAAGTTC-3′ SYBR Green
Col 3A1 5′-TGAAGATGTCGTTGATGTGCAG-3′ 5′-GCAGTGGTATGTAATGTTCTGGGAG-3′ SYBR Green
Tgfb 5′-GGACTCTCCACCTGCAAGACC-3′ 5′-GGATGGCTTCGATGCGC-3′ SYBR Green
CDKN1A 5′-GTGGCCTTGTCGCTGTCTT-3′ 5′-GCGCTTGGAGTGATAGAAATCTG-3′ SYBR Green
P27 5′-GCAAAACAAAAGGGCCAACA-3′ 5′-GGGCGTCTGCTCCACAGT-3′ SYBR Green
CCND1 5′-ACAACTCTATCCGGCCCGA-3′ 5′-GGCCAGGTTCCACTTGAGC-3′ SYBR Green
CCNE1 5′-GTTGGCTGCTTAGAATTTCCTTATG-3′ 5′-ACTGATAACCTGAGACCTTCTGCAT-3′ SYBR Green
Gapdh 5′-GGCAAATTCAACGGCACAGT-3′ 5′-AGATGGTGATGGGCTTCCC-3′ SYBR Green
Ccl2 5′-TGGCTCAGACAGATGCAGT-3′ 5′-ATTGGGATCATCTTGCTGGTG-3′ SYBR Green
Acta2 5′-CGTGGATGCCCGCTGA-3′ 5′-TACGCTTCCGCTGCCC-3′ SYBR Green
Fibronectin 5′-GATGGAATCCGGGAGCTTTT-3′ 5′-TGCAAGGCAACCACACTGAC-3′ 5′-CCGGCCTGAGGCCCTGCAG-3′
Pdgfb 5′-CCATCCGCTCCTTTGATGAT-3′ 5′-AAGTCCAGCTCAGCCCCAT-3′ 5′-CGCCTGCTGCACAGAGACTCCGTA-3′