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. Author manuscript; available in PMC: 2018 Apr 23.
Published in final edited form as: Nat Chem Biol. 2017 Oct 23;13(12):1274–1279. doi: 10.1038/nchembio.2499

Figure 4. PQ negative-selection CRISPR-based screen.

Figure 4

(a) The gene scores as differentially required for the top 25 genes following treatment with 25 μM PQ. The copper transporter gene SLC31A1 and the copper-dependent antioxidant gene SOD1 were among the top genes identified. (b) Wild-type Jurkat cells were treated with varying concentrations of the copper-chelating agent 2,3,2-tetraamine (Tet; 5) in the presence or absence of 25 μM PQ for 7 days and the percentage of cellular viability was assessed. Error bars represent SEM (n=4 independent experiments). **, p < 0.01 compared to the 0 μM Tet condition within each PQ treatment group.

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