Table 2.
Accuracy, precision, and linearity of intra-day calibration standards (n=3)
| Calibration standards (fmol) | 62.5 | 125 | 250 | 625 | 2500 | slope | rˆ2 | y-intercept | |
|---|---|---|---|---|---|---|---|---|---|
| dATP | |||||||||
| Mean ± SD | 67.2 ± 13.3 | 126.0 ± 25.6 | 259.3 ± 7.5 | 606.5 ± 49.8 | 2503.5 ± 10.7 | 5.46E-04 ± 1.32E-05 | 0.9999 ± 0.0009 | −0.0085 ± 0.009 | |
| CV (%) | 19.8% | 20.3% | 2.9% | 8.2% | 0.4% | ||||
| Accuracy (%) | 107% | 101% | 104% | 97% | 100% | ||||
| dGTP | |||||||||
| Mean ± SD | 60.8 ± 15.0 | 133.6 ± 6.5 | 239.6 ± 22.1 | 628.8 ± 36.4 | 2499.7 ± 6.8 | 1.91E-04 ± 2.19E-05 | 1.0000 ± 0.0002 | −0.0033 ± 0.0123 | |
| CV (%) | 24.7% | 4.8% | 9.2% | 5.8% | 0.3% | ||||
| Accuracy (%) | 97% | 107% | 96% | 101% | 100% | ||||
| dCTP | |||||||||
| Mean ± SD | 62.3 ± 11.7 | 105.8 ± 5.7 | 258.9 ± 24.2 | 638.9 ± 11.6 | 2496.6 ± 1.0 | 4.31E-04 ± 2.71E-05 | 1.0000 ± 0.0002 | −0.0022 ± 0.0122 | |
| CV (%) | 18.8% | 5.3% | 9.3% | 1.8% | 0.1% | ||||
| Accuracy (%) | 111% | 89% | 103% | 100% | 96% | ||||
| dTTP | |||||||||
| Mean ± SD | 76.4 ± 1.4 | 123.4 ± 21.4 | 272.2 ± 17.4 | 582.3 ± 35.7 | 2508.2 ± 7.0 | 4.75E-04 ± 2.80E-05 | 0.9999 ± 0.0009 | −0.0113 ± 0.0047 | |
| CV (%) | 1.9% | 17.3% | 6.4% | 6.1% | 0.3% | ||||
| Accuracy (%) | 122% | 99% | 109% | 93% | 100% | ||||