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. 2017 Oct 24;95(10):933–942. doi: 10.1038/icb.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Perturbation in chemokine expression in the Il7r^−/− thymus. (a) Whole homogenized thymus was used as a template for quantitative reverse transcription-PCR (qRT-PCR) to measure the amount of chemokine mRNA produced in the adult WT versus Il7r^−/− thymus. To account for the fivefold greater number of TECs in the Il7r^−/− thymus versus the WT thymus, results were normalized to EpCAM mRNA levels. (b) TECs were sorted based on expression of EpCAM, and Ly51 (cTECs) or UEA-1 (mTECs). cDNA was generated from sorted TEC subsets and used as template for qRT-PCR. n=3. Values were normalized to β-actin mRNA levels. Data are representative of at least two separate experiments. Graphs depict means±s.e.m. Statistical significance was calculated using a t-test; ***P<0.005, **P<0.01, *P>0.05, ns=nonsignificant.