Figure 6.

Increase of Il7r^−/− donor-derived thymic DC cell numbers by the provision of WT donor cells. Lethally irradiated CD45.1⁺ × CD45.2⁺ F1 hybrid mice were reconstituted with whole BM from CD45.1⁺ WT mice (S-WT), CD45.2⁺ Il7r^−/− (S-KO) mice or a 1:1 mix of each (mixed). The thymus was analyzed by manual counting and flow cytometry 3 weeks later. (a) Numbers of CD45⁺ donor cells of the indicated genotypes per thymus in the single and mixed chimeras. (b) Percentages of total DCs within the total donor populations of the indicated genotypes (WT or KO) in the single and mixed chimeras. (c) Numbers of pDCs, CD11b⁺ cDCs and CD11b⁻ cDCs per thymus of the indicated genotypes (indicated below the graph) in the single and mixed chimeras (indicated above the graph). (d) Comparison of the sum of WT and KO cell numbers in the mixed chimeras to the WT or KO cell numbers in the single chimeras. The graphs depict the mean±s.e.m. Statistical significance was calculated using a t-test. ns, nonsignificant, n=5. Data are representative of two separate experiments.