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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Circulation. 2017 Oct 3;136(21):2037–2050. doi: 10.1161/CIRCULATIONAHA.117.028202

Figure 7. Transcript and protein levels of ion channel genes and fibrosis in control and HFpEF rats.

Figure 7

A. Quantitative reverse transcriptase PCR showed decreased expression of Kcnd2 and Kcnd3 mRNAs in HFpEF hearts compared to control hearts. Y axis indicates log (fold change). HFpEF rats n=4. B. Western blot of Kv4.2 showed no expression differences in the two groups. C. Kv4.3 expression was decreased in HFpEF rats compared to control rats. D. Kv4.2/GAPDH ratio did not differ between the two groups. n=3 rats each group. E. Kv4.3/GAPDH ratio was markedly decreased in HFpEF rats compared to control rats. n=3 rats each group. F. Representative Masson’s trichrome-stained sections of control and HFpEF hearts. G. Increased fibrosis in HFpEF hearts compared to controls. Error line indicates mean and standard deviation. * denotes p < 0.05 and ** denotes p < 0.001. Multiple t-tests were performed using step-down bootstrap sampling to control the familywise error rate at 0.05 for 7A, D and E, and unpaired t-test was used for 7G.