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Automated dual sample analysis by VAL-DESI-MS/MS: (A) TIC mass abundance trace obtained from the analysis; (B) m/z 205➔ 188 extracted mass abundance trace for detection of Trp from the Trp-containing urine sample (2.0 μM Trp); and (C) m/z 177➔ 160 extracted mass abundance trace for detection of 5-HT from 5-HT-containing urine sample (0.50 μM 5-HT). Experimental conditions were as in Fig 2.
