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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Chem Commun (Camb). 2017 Nov 21;53(93):12532–12535. doi: 10.1039/c7cc07389e

Fig 4. APBA coumarin derivatives efficiently label S. aureus membranes without washing.

Fig 4

(a) Schematic two-step labelling process involving 1) D-Dap-semicarbazide incorporation into peptidoglycan followed by 2) APBA coumarin conjugation to form a stable diazaborine. (b) S. aureus incubated with 0 μM, 100 μM or 500 μM D-Dap-Semicarbazide for four hours followed by 100 μM AB21, AB22, AB24 or FPBA-FITC incubation for 2 hours. AB21 and AB22 showed dose dependent bacterial membrane labelling, whereas AB24 displayed no fluorescence labelling and FPBA-FITC gave significant background staining.