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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Immunol Cell Biol. 2017 Aug 22;95(10):960–965. doi: 10.1038/icb.2017.63

Figure 3. THP binds mouse Siglec-E, and regulates urinary neutrophil populations in mice.

Figure 3

(A)Plate-based binding assay of immobilized mouse THP with mouse Siglec-E-Fc and visualized with PE anti-human IgG Fc antibody. Percent (B) or total (C) Ly6G+/CD11b+ cells in mouse urine collected 24 h post-treatment with LPS, and quantified via flow cytometry. Experiments were performed independently three times, and combined results are shown. Data represent the mean and SEM of three independent experiments performed in technical triplicate, n = 3/group (A). Circles represent individual mice and lines represent the median and IQ ranges of each group, n = 12-16/group (B, C). Data were analyzed using Mann-Whitney test, two-tailed (B,C), or using Student's unpaired two-tailed t-test (A). *P < 0.05, **P < 0.01, and ****P < 0.0001 represent statistical significance, or n.s. represents non-significant (P > 0.05).