Table 2.

Autocorrelation data under the various ligated conditions. The average autocorrelation decay of the single molecule FRET data was fit to a three-term exponential decay, resulting in short, intermediate and long timescale decays for each condition.

Ligand	Short timescale τ1 (ms)	Amplitude (%)	Intermediate timescale τ2 (ms)	Amplitude (%)	Long timescale τ3 (ms)	Amplitude (%)	Weighted average lifetime τ (ms)
Apo	8 ± 1	46.4 ± 0.7	170 ± 10	30 ± 1	860 ± 60	23 ± 2	250 ± 20
Glu-Gly	9 ± 3	37 ± 1	82 ± 6	24.3 ± 0.9	650 ± 10	38.9 ± 0.7	275 ± 6
Gly-Gly/Zn2+	13 ± 1	39 ± 1	150 ± 10	34 ± 2	640 ± 40	26 ± 2	220 ± 20
Glu-Gly/MK-801, 50 μM	20 ± 1	32 ± 1	—	—	560 ± 6	66.4 ± 0.6	378 ± 8