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Enrichment of peptides containing cysteine using Thiopropyl Sepharose 6B resin. Peptides from the yeast whole-cell lysate are incubated with the resin. Peptides containing cysteine are bound to the resin through disulfide bonds while unbound peptides are removed. Enriched peptides are cleaved with DTT, and then alkylated with iodoacetamide prior to LC-MS/MS analysis.
