Table 1.
cTnI(WT)
|
cTnI(1–167)
|
cTnI(1–193)
|
||||
---|---|---|---|---|---|---|
1.9µm | 2.2µm | 1.9µm | 2.2µm | 1.9µm | 2.2µm | |
Tmin (mN mm−2) | 1.00±0.06 | 3.74±0.26* | 1.24±0.19 | 3.34±0.09* | 0.67±0.03 | 2.15±0.29*†# |
Tmax (mN mm−2) | 13.25±1.75 | 31.25±0.62* | 28.42±1.59† | 47.96±1.37*† | 13.38±1.07# | 25.72±0.73*# |
Tdev (mN mm−2) | 12.25±1.50 | 27.51±0.54* | 27.18±2.21† | 44.62±1.29*† | 12. 71±1.1# | 23.5 7±0.69*# |
pCa50 | 5.29±0.01 | 5.37±0.02* | 5.44±0.01† | 5.46±0.02† | 5.39±0.01†# | 5.48±0.02*† |
nH | 4.64±0.21 | 6.18±0.11* | 8.15±0.39† | 7.27±0.21† | 4.68±0.15# | 5.78±0.13*# |
n fibers | 6 | 6 | 7 | 7 | 6 | 6 |
Tmin, absolute tension value at pCa 8.0; Tmax, absolute tension value at pCa 4.8; Tdev, Ca2+-activated developed tension (Tmax- Tmin); pCa50 and nH represent fit parameters to a 3-parameter Hill equation.
Effect of SL for each truncation or wild-type; P <0.05
Truncation effect compared to cTnI(WT) at same SL; P <0.05
Difference between cTnI(1–193) and cTnI(1–167) at same SL; P <0.05