Figure 7.

Involvement of p-CREB in CFTR-regulated glucagon production in αTC1-9 cells. (A,B) Western blot with quantitation (right) for CFTR, p-CREB, and CREB (A), and proglucagon, Pax6 and PC2 (B) in αTC1-9 cells transfected with the empty vector plasmid (pVector) or pEGFP conjugated with full length CFTR (pCFTR). GAPDH or β-tubulin was used for loading control. ^*P < 0.05, t-test. ns, no significant difference. n is shown in each column. (C) qPCR of glucagon and PC2 in αTC1-9 cells after transfected with pVector or pCFTR for 48 h. ^**P < 0.01, ^***P < 0.001, t-test, ns, not significant. The experiments were repeated for three times.