Figure 3.

The reduction of SP cells in NCOR1 cKO^Cd4 mice is T cell-intrinsic. Flow cytometry analysis of CD45.1 and CD45.2 expression (upper panels) on thymocytes (a) and splenocytes (b) isolated from BM chimeric CD45.1⁺ mice that received either Ncor1 ^f/f (WT) or Ncor1 ^f/f Cd4-Cre (NCOR1 cKO^Cd4) CD45.2⁺ BM cells that had been mixed at a 1:1 ratio with wild-type (WT, CD45.1⁺) BM cells. The lower panels in (a) show CD24 and TCRβ expression on thymocytes pre-gated on CD45.1⁺ and CD45.2⁺ subsets, while the lower panels in (b) depicts TCRβ expression on splenocytes pre-gated on CD45.1⁺ and CD45.2⁺ subsets. Data are representative of 9–10 mice analyzed in 3 independent experiments.