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. 2017 Nov 17;8:2272. doi: 10.3389/fmicb.2017.02272

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Copyright © 2017 Chimetto Tonon, Thompson, Moreira, Garcia, Penn, Lim, Berlinck, Thompson and Thompson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplification of target DNA in a background of closed related non-target. (A) Target and non-target DNA concentration used in the mixed reaction. (B) Copies detected by qPCR. All non-target DNA had 10-fold excess compare with target DNA. Target DNA i.e. (total Vibrio, V. harveyi, and V. coralliilyticus) were diluted at least 1:100 while non-target (i.e., E. coli, V. campbellii, V. communis, V. tubiashii, and V. neptunius) were diluted 1:10. Similarities of 16S rRNA between target and non-target are represented in line.