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. 2017 Nov 17;8:2272. doi: 10.3389/fmicb.2017.02272

Table 2.

Primer information.

CODE Sequence 5′-3′ Tm (°C) Amplicon (bp) Gene target Phylogenetic target
567F GGC GTA AAG CGC ATG CAG GT 61.4 114 16S rRNA (Thompson et al., 2004) Vibrio spp.
680R GAA ATT CTA CCC CCC TCT ACA G 54.3
Vc pyrH F CAA CTG GGC AGA CGC AAT CCG TGA GT 64.8 166 pyrH (this study) V. coralliilyticus
Vc pyrH R CGT AAA TAC GCC ATC AAC TTT TGT C 54.9
Vh pyrH F CTT GCA ACG GTA ATG AAC GGT TTG GCA 61.9 171 pyrH (this study) V. harveyi
Vh pyrH R AGT ACC TGC AGA GAA GAT TAC CAC T 57.1