Table 4.
Complete list of proteins and encoding genes of L. plantarum S2T10D (butyrogenic strain) and L. plantarum O2T60C (non-butyrogenic strain) involved in the FASII-Pls pathway, together with results obtained from SNPs meaning (S2T10D vs. O2T60C) and prediction of the functional impact of mutation by means of PROVEAN.
| Clusters | Proteins | Gene names* | E.C. no | Locus tag | O2T10D vs. S2T10D | |||
|---|---|---|---|---|---|---|---|---|
| S2T10D (butyrogenic) | O2T60C (non-butyrogenic) | nsSNPs | PROVEAN prediction (cutoff = −2.5) | Amino acids mutations (score) | ||||
| I | 3-oxoacyl-ACP synthase III (FabH) | fabH1 | 2.3.1.180 | BBF95_00504 | BBA85_01856 | 1 | Neutral | |
| Biotin carboxyl carrier protein (AccB) | accB1 | 6.4.1.2 | BBF95_00505 | BBA85_01857 | ||||
| Biotin carboxylase subunit (AccC) | accC1 | 6.4.1.2 | BBF95_00506 | BBA85_01858 | 3 | Neutral | ||
| Carboxyl transferase subunit β (AccD) | accD1 | 6.4.1.2 | BBF95_00507 | BBA85_01859 | 2 | Neutral | ||
| Carboxyl transferase subunit α (AccA) | accA1 | 6.4.1.2 | BBF95_00508 | BBA85_01860 | 3 | Neutral | ||
| II | Phosphate acyltransferase (PlsX) | plsX | 2.3.1._ | BBF95_00619 | BBA85_01666 | |||
| Acyl carrier protein (ACP) | acpA1 | / | BBF95_00620 | BBA85_01667 | ||||
| III | 3-hydroxyacyl-ACP dehydratase (FabZ) | fabZ1 | 4.2.1.59 | BBF95_00945 | BBA85_03004 | |||
| 3-oxoacyl-ACP synthase III (FabH) | fabH2 | 2.3.1.180 | BBF95_00946 | BBA85_03005 | ||||
| Acyl carrier protein (ACP) | acpA2 | / | BBF95_00947 | BBA85_03006 | ||||
| Malonyl CoA-ACP transacylase (FabD) | fabD | 2.3.1.39 | BBF95_00948 | BBA85_03007 | 1 | Neutral | ||
| 3-oxoacyl-ACP reductase (FabG) | fabG1 | 1.1.1.100 | BBF95_00949 | BBA85_03008 | 1 | Neutral | ||
| 3-oxoacyl-ACP synthase II (FabF) | fabF | 2.3.1.179 | BBF95_00950 | BBA85_03009 | ||||
| Biotin carboxyl carrier protein (AccB) | accB2 | 6.4.1.2 | BBF95_00951 | BBA85_03010 | ||||
| (3R)-hydroxymyristoyl-ACP dehydratase (FabZ) | fabZ2 | 4.2.1.59 | BBF95_00952 | BBA85_03011 | ||||
| Biotin carboxylase subunit (AccC) | accC2 | 6.3.4.14 | BBF95_00953 | BBA85_03012 | ||||
| Carboxyl transferase subunit β (AccD) | accD2 | 6.4.1.2 | BBF95_00954 | BBA85_03013 | 2 | Deleterious | Q231K (−3.255) | |
| Carboxyl transferase subunit α (AccA) | accA2 | 6.4.1.2 | BBF95_00955 | BBA85_03014 | ||||
| Enoyl-ACP reductase (FabI) | fabI | 1.3.1.9 | BBF95_00956 | BBA85_03015 | ||||
| Holo-ACP synthase (ACPS) | acps | 2.7.8.7 | BBF95_00957 | BBA85_03016 | 1 | |||
| Acyl-ACP thioesterase | lp_0708 | 3.1.2.14 | BBF95_00554 | BBA85_01904 | ||||
| Biotin carboxyl carrier protein (AccB) | accB3 | 6.4.1.2 | BBF95_00112 | BBA85_02896 | ||||
| 3-oxoacyl-ACP reductase (FabG) | lp_0159 | 1.1.1.100 | BBF95_01809 | BBA85_02754 | ||||
| 3-oxoacyl-ACP reductase (FabG) | fabG2 | 1.1.1.100 | BBF95_01940 | BBA85_00593 | ||||
| Putative enoyl-ACP reductase (FabI2) | lp_0912 | 1.3.1.9 | BBF95_02498 | BBA85_01269 | 2 | Neutral | ||
*genes names are referred to L. plantarum WCFS1.