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. 2017 Nov 21;7:15895. doi: 10.1038/s41598-017-16291-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Limiting effect of formamide on fission yeast proliferation. (a) Five-fold serial dilutions were spotted on YES plates for 3 days at 30° in the presence of indicated formamide concentrations (v/v). (b) Growth curves of S. pombe in rich liquid media in the presence of 0%, 1%, 2% and 3% respectively. Normalized values by initial number of cells and averaged from two biological repeats are plotted (left). Duplication time in each concentration was calculated by interpolating from average logarithmic equations (right). Logarithmic lines in grey correlation coefficient and line slopes are also indicated. (c) DAPI/Calcofluor staining of cells incubated for 12 hours from a starting density of 1.5 × 10⁶ cells/ml at the respective concentrations of formamide. Cells in the absence of formamide reach stationary phase, becoming smaller as they starve; while in the presence of formamide over the same time, cell cultures do not reach stationary phase since growth rate is delayed. Scale bar: 10 µm.