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. 2017 Nov 21;7:15895. doi: 10.1038/s41598-017-16291-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global characterization of fsm mutants. Clustering of fsm alleles by other sensitivities to widespread conditions. A colour code was assigned for three degrees of sensitivity in comparison to wild type standards onto the same plate: Solid black for severe, light grey for intermediate and white for no sensitivity. An example of each is shown on top. Fsm strains can be grouped in three distinct clusters. About one third of formamide sensitive mutants (cluster III) show no sensitivity to other conditions tested. Right panel ticks most obvious morphology and cell cycle phenotypes for each mutant based on DAPI/calcofluor co-staining after 4 hours in the presence of formamide. Numbers in categories 4 and 5 represent septation percentage as compared to 8% found in wild type control and most of the mutants in the same condition. Representative phenotype cartoons are numbered on top to be referred to in Fig. 4 and Supplementary Figure 3.