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. 2017 Nov 21;7:15918. doi: 10.1038/s41598-017-15139-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of xenograft CRC tumor growth and manifestation of EMT by stable knockdown of USP47. BALB/C nude mice were injected subcutaneously with shControl- or shUSP47-transfected DLD-1 cells (N = 5 mice/group) and sacrificed after 4 weeks. (a) Representative images of xenografts derived from shCtrl- and shUSP47-transfected DLD-1 cells. (b,c) Changes in the volume (b) and the weight (c) of xenograft tumors. Results are representative of three independent experiments. (d) Protein expression levels of USP47 and EMT markers in xenografts derived from USP47-deficient and control DLD-1 cells were examined by Western blot analysis. (e) Representative immunofluorescence images of tumors derived from shControl- or shUSP47-treated DLD-1 cells. Paraffin sections of the tumors were stained for USP47, PCNA, E-cadherin, N-cadherin, vimentin, and Snail. Scale bar = 100 μm. Statistical significance was assessed using the two-sided Student’s t-test; *P < 0.05, **P < 0.01, and ***P < 0.001.