Figure 1.
T. citrinoviride conidiation. (a) Ancestral T. citrinovirde grows as mycelia balls in malt extract media with no transferable conidia production in 30 hours (clear liquid), while selected T. citrinovirde T-6 strain produced large numbers of conidia (>106 conidia per mL, green) in malt extract media, resulted in a cloudy liquid culture. (b) No fresh conidia production of the ancestral population (A) in 24 hours, while one of ten 45th derived populations (numbers) and six of ten 85th derived populations (numbers) produced large numbers (103–106 per mL) of conidia in liquid cultures without filter paper strips in 24 hours. (c) Flow cytometry dot plots show no conidia in the ancestor liquid cultures, but large numbers of conidia in the T-6 liquid cultures. (d) Upon transfer to fresh media, condia density declines sooner in T-6 than the ancestor, as a consequence of more rapid germination. Condia formation starts far sooner in T-6, yielding about 106 conidia per mL in by 24 hours, while no conidia production occurs in the ancestor by that time.