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. 2017 Oct 29;80(12):1723–1732. doi: 10.1007/s10337-017-3421-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Overview of 4-BNMA derivatization scheme. Simplified 4-BNMA derivatization of generic carboxylic acid is shown on the left. Dotted line represents location of fragmentation in MRM analysis. The right depicts the ESI + precursor M + H isotope patterns for representative doublet from a mono-(pyruvate), triplet from a di-(fumarate) and quartet from a tri-(citrate) 4-BNMA derivatized carboxylic acid and the isotope pattern from the highest intensity product ion signal resulting from a resonance stabilized ion fragment from 4-BNMA