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. 2017 Jun 1;183(4):1414–1425. doi: 10.1007/s12010-017-2508-2

Table 1.

The amounts of estimated metabolites in extracts from biomass of S. lateriflora cultured in vitro on MS medium variants under different hormonal combinations and light conditions after 6 weeks of culture [mg 100 g-1 DW ± SD] (− indicates not detected)

Light conditions Medium variant
BAP/NAA
[mg l−1]
Estimated metabolites [mg 100 g−1 DW]
Baicalein Wogonin Baicalin Wogonoside 3,4-Dihydroxy-phenylacetic acid Verbascoside
Red 1.0/1.0 7.29 ± 0.62 24.12 ± 3.37*** 428.05 ± 22.11 60.63 ± 6.01 22.03 ± 1.91 123.52 ± 11.13#,##
2.0/2.0 4.88 ± 1.43 21.82 ± 3.21*** 367.76 ± 51.43 50.35 ± 5.18 12.01 ± 1.89 75.48 ± 0.00
3.0/1.0 7.70 ± 1.24 49.42 ± 11.54*,**,#,## 420.31 ± 57.72 51.90 ± 6.63 30.71 ± 6.65 128.97 ± 31.63#,##
0.5/2.0 7.09 ± 1.16 16.04 ± 1.40*** 192.98 ± 89.33 26.23 ± 13.58 12.48 ± 2.84 57.00 ± 13.79*,***
1.0/0.5 5.91 ± 1.13 22.49 ± 6.79*** 303.25 ± 89.50 48.29 ± 13.40 29.85 ± 9.20 60.49 ± 15.02*,***
Blue 1.0/1.0 24.06 ± 5.57 886.44 ± 127.79*** 293.84 ± 53.34 16.08 ± 2.54 125.67 ± 18.5
2.0/2.0 32.82 ± 5.06 635.34 ± 138.40 118.42 ± 21.94 24.95 ± 4.37 93.31 ± 28.27
3.0/1.0 25.93 ± 1.60 491.65 ± 13.80* 116.50 ± 6.72 11.49 ± 3.05 54.51 ± 6.08
0.5/2.0 39.57 ± 12.25 704.52 ± 100.05 172.93 ± 15.56 24.45 ± 7.85 169.15 ± 62.45
1.0/0.5 28.56 ± 11.49 761.08 ± 101.87 187.55 ± 92.91 10.66 ± 3.23 108.15 ± 15.64
UV-A 1.0/1.0 14.89 ± 2.35 395.65 ± 109.64 59.20 ± 16.79 17.85 ± 2.76 64.41 ± 11.44
2.0/2.0 14.70 ± 6.00 266.26 ± 17.04 56.10 ± 16.81 17.78 ± 4.47 110.00 ± 27.98***,#,##
3.0/1.0 18.37 ± 6.10 169.57 ± 22.35 38.35 ± 4.38 11.77 ± 4.89 47.96 ± 16.44**
0.5/2.0 14.33 ± 5.70 263.75 ± 93.99 46.26 ± 14.81 21.81 ± 7.98 51.04 ± 3.86**
1.0/0.5 15.11 ± 1.29 358.83 ± 112.35 50.68 ± 14.01 18.92 ± 5.67 40.23 ± 8.68**
Darkness 1.0/1.0 5.68 ± 1.04*** 20.85 ± 2.03*** 13.37 ± 2.51**,***,#,## 14.68 ± 17.22***,#,##
2.0/2.0 3.59 ± 1.12***,## 20.29 ± 6.51*** 101.86 ± 24.50*,***,## 41.77 ± 9.80
3.0/1.0 28.31 ± 5.47*,**,#,## 33.36 ± 1.25*,** 205.95 ± 0.48*,**,# 109.41 ± 0.26*
0.5/2.0 8.33 ± 0.78*** 27.31 ± 3.24 103.57 ± 10.70*,***,## 111.39 ± 14.64*
1.0/0.5 14.31 ± 2.10**,*** 26.80 ± 1.53 260.37 ± 46.13*,**,# 122.94 ± 41.26*
White (control) 1.0/1.0 15.24 ± 1.94 29.33 ± 5.45 592.63 ± 158.17 142.98 ± 45.28 33.56 ± 4.8*** 88.67 ± 19.14
2.0/2.0 10.04 ± 4.00 33.47 ± 7.45 317.92 ± 106.0 61.79 ± 31.95 19.49 ± 2.70 100.64 ± 18.89
3.0/1.0 8.19 ± 3.10 14.53 ± 6.00 306.64 ± 96.78 58.66 ± 20.10 6.07 ± 2.27* 51.31 ± 24.20
0.5/2.0 94.82 ± 43.38 40.09 ± 13.13 484.50 ± 157.19 107.51 ± 26.01 24.43 ± 8.68 130.20 ± 42.57
1.0/0.5 84.21 ± 33.01 24.70 ± 6.32 792.17 ± 136.49 141.65 ± 18.85 18.54 ± 4.49 70.29 ± 12.91

Statistically significant differences: *p < 0.05 vs. MS variant 1.0/1.0; **p < 0.05 vs. MS variant 2.0/2.0; ***p < 0.05 vs. MS variant 3.0/1.0; # p < 0.05 vs. MS variant 0.5/2.0; ## p < 0.05 vs. MS variant 1.0/0.5