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Purification of FtFBPaseII. Purification of FtFBPaseII was carried out with his-tag nickel affinity purification procedures, which resulted in highly pure protein of approximately 37 kDa, as seen in Lane 1. Lane 8 contains PageRuler Prestained Protein Ladder of indicated size bands in kDa. Lanes 3–6 contain non-binding washes. Lane 7 is the urea-soluble portion of the pellet
