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. 2017 May 25;183(4):1439–1454. doi: 10.1007/s12010-017-2512-6

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — SEC chromatogram for FtFBPaseII. A main peak at about 190 mL corresponds to the FtFBPaseII tetramer (a). A small peak at 350 mL might be the diluted monomeric form of FBPase protein or other buffer ingredients. The peak maximum of first peak was about 650 mAu. SEC was repeated for FtFBPaseII protein for molecular weight estimation (b). The chromatogram indicated the presence of dimeric and tetrameric species (63.8 and 124.9 kDa, respectively) by calculation from a calibration curve using molecular weight standards, thyroglobulin (bovine) 670 kDa, gamma globulin (bovine) 158 kDa, ovalbumin (chicken) 44 kDa, myoglobulin (horse) 17 kDa, and vitamin B12 1350 Da. Calibration curve can be found in previous publication [6]. Peak maximums are about 52 and 93 mAu