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Composition and position of ultrafilter. The ultrafilter was composed of two serially positioned devices with polysulfone membranes [MediSulfone, Medica, Medolla (MO), Italy] of 2.0 and 1.0 m2, respectively. Characteristics of the ultrafilter were: bacteria retention capacity >1010 CFU/ml (Brevundimonas diminuta), viruses retention capacity >108 (PhiX 174), endotoxin retention capacity >105 EU/ml
