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. 2017 Nov 21;7:15976. doi: 10.1038/s41598-017-14159-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of when protein concentrate (WPC), rapamycin, and their combination on Nrf2 translocation in MDA-MB-231 cells. (a) Representative western blots showing the effect of 48 hours of exposure to 10 mg/mL WPC, 1 μM rapamycin, and their combination on cytosolic and nuclear Nrf2 protein levels. α-tubulin and lamin B were used as loading controls for cytosolic and nuclear fractions, respectively. (b) Bar graphs showing relative nuclear/cytosolic Nrf2 ratios. Data represent the means ± SD from three experiments. *P < 0.05.