Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 21;7:15976. doi: 10.1038/s41598-017-14159-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Whey protein concentrate (WPC)-induced reduction of Nrf2 nuclear accumulation and mTORC1 activation are disrupted by GSK3β siRNA. MDA-MB-231 cells were transfected with non-specific siRNA or GSK3β-targeting siRNA and treated for 48 hours with 10 mg/mL WPC. (a) Representative western blots showing GSK3β protein levels. β-actin was used as a loading control. (b) Representative western blots showing cytosolic and nuclear levels of Nrf2. Lamin B and α-tubulin were used as loading controls for nuclear and cytosolic fractions, respectively. (c) Bar graphs showing relative nuclear/cytosolic Nrf2 ratios. (d) Representative western blots demonstrating levels of p-mTOR (Ser2448) and mTOR. β-actin was used as a loading control. (e) Bar graphs displaying relative p-mTOR/mTOR ratios. Data represent the means ± SD from three experiments. *P < 0.05; n.s.: not significant.