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. 2017 Nov 22;8:1681. doi: 10.1038/s41467-017-01715-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Mapping the CaM interaction in AKAP79 using an alphascreen—peptide scanning procedure. a Principle of the alphascreen assay. In this case, illumination at 680 nm leads to emission between 520 and 620 nm if donor and acceptor beads are co-localized through interaction of GST-AKAP79 (1–153) and biotin-CaM. b Alphascreen recordings of biotin-CaM association with GST or GST-AKAP79 (1–153). Recordings were performed either with or without Ca²⁺ (n = 6). c Position of 20-mer peptides in the N-terminus of AKAP79 used for scanning. d Changes in alphascreen signal between biotin-CaM and GST-AKAP79 (1–153) are shown upon inclusion of each of the 20-mer peptides outlined in panel c. Peptides were added at 100 nM (n = 5). Error bars show s.e.m.