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. 2017 Nov 21;7:15929. doi: 10.1038/s41598-017-16175-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-30e regulates cell proliferation, migration, invasion and increases chemosensitivity of MDA-MB-231 cells to paclitaxel by inhibiting its target IRS1. (a) Overexpression of miR-30e arrested cell proliferation, but this was rescued upon coexpression of exogenous IRS1 in MDA-MB-231 cells. (b) Cells were treated as above. A sterile 200 μl pipette tip was used to scratch the cells to form a wound. The wound gaps were photographed (top) and measured (bottom). Forced expression of miR-30e also markedly reduced the wound-healing rate, and overexpression of IRS1 reverses the inhibitory effects of miR-30e. (c) MiR-30e overexpression decreased cell invasion in MDA-MB-231 cells. Cells were transfected with miR-30e followed by IRS1 transfection. All cells were subjected to a Matrigel invasion assay. (d) MDA-MB-231 cells stably expressing miR-NC or miR-30e were pretreated with various concentration of paclitaxel for 48 h, and subjected to CCK8 Assay. (e,f) MDA-MB-231 cells stably expressing miR-NC, miR-30e or miR-30e forced expression of IRS1 were pretreated with 4 nM of paclitaxel for definite time points, and subjected to CCK8 Assay, apoptosis analysis by flow cytometry. (g) MDA-MB-231cells stably expressing miR-NC or miR-30e were transfected with 2 μg pCMV6 vector or pCMV6–IRS1 plasmid and cultured with or without paclitaxel. After 72 h, the relative caspase-3 activities were determined. Data represent mean ± SD. of 3 replicates. * or ^# indicated significant difference at P < 0.05. **Indicated significant difference at P < 0.01. *Indicates significant difference compared to control; ^#indicates significant difference compared to miR-30e plus IRS1 treatment.