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. 2017 Nov 21;7:15964. doi: 10.1038/s41598-017-16297-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolic measurements before and after a 3 min chase-and-exhaust protocol. ABC) Mass-specific oxygen consumption (VO₂) in adult individuals of (a) Red Junglefowl (Gallus gallus, Gg, N = 5), (b) Chilean Tinamou (Nothoprocta perdicaria, Np, N = 10) and (c) Ornate Tinamou (Nothoprocta ornata, No, N = 6). Open symbols show the data for the 60 min baseline measurements and closed symbols show the data for the 90 min following the chase protocol. (d) Excess post-exercise oxygen consumption (EPOC) obtained by integrating the pre- and post-curves shown as ABC. (e) Plasma lactate levels obtained using the same protocol in a separate group of individuals (Gg N = 6, Np N = 8, No N = 16). Data in A–C presented as mean and standard deviations. Data in D–E presented as mean and 95% confidence intervals with individual data points shown. For statistical analysis we used general linear modeling (GLM) considering species (D) and species/treatment (E) as factors followed by Tukey posthoc test with a customary fiduciary significant level of p < 0.05 (shown as dissimilar letters) in Minitab 17. No statistical analysis was performed for panels ABC because the integrated response is considered in panel (d).