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. 2017 Nov 21;7:15946. doi: 10.1038/s41598-017-16290-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE and SDS-CGE analyses of purified recombinant PICP and PIIICP. (a) Non-reducing and reducing SDS-PAGE analyses of the purified recombinant PICP and PIIICP. The arrows dictate the eluted position of indicated proteins. The number below the band indicates the relative value of band intensity of each band to that of PICP heterotrimer band in the non-reducing conditions. (b) Gel-like image of SDS-CGE analysis of the purified recombinant PICP and PIIICP using a Protein 230 assay kit. In non-reducing conditions, PICP and PIIICP appeared mainly as a single band at the expected size. In reducing conditions, PICP and PIIICP showed two bands for PICPα1 and PICPα2 and a single band for PIIICPα1, respectively. (c) Electropherogram of SDS-CGE analysis of the recombinant PICP and PIIICP shown in (b). Significant peaks are marked with black arrow heads, with the calculated molecular weights. The inset table below shows the precise quantity and ratio of each peak area in the electropherogram. The raw images of SDS-CGS analysis are shown in Supplementary Fig. 5.