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. 2017 Nov 21;7:15894. doi: 10.1038/s41598-017-15893-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of RNA interference on the Bactrocera dorsalis male flies’ mortality, BdorOBP2 expression in antennae and responsiveness to ME. (A,B) Mortality rate of male flies in the dsBdorOBP2 treatment in the 24 h and 48 h bioassays was calculated. Normal control males were injected with the same amount of dsGFP and Buffer. Blank control groups (CK) were normally reared. (C,D) The qRT-PCR analysis of BdorOBP2 expression after BdorOBP2 was silenced by RNAi. (E,F) The attractive effect of ME to males after BdorOBP2 was silenced by RNAi. Different letters among the columns indicate significant differences (ANOVA, P < 0.05). Five biological replicates were performed. Bars represent mean ± SE values.