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. 2017 Nov 21;7:15911. doi: 10.1038/s41598-017-16092-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-PCR (a) and qRT-PCR (b) results of relative gene expression of 9 interested putative secretory proteins of S. avenae salivary glands in different tissues. The display for each unigenes are cropped figures from the gels. The full-length gels are presented in Supplementary Figure 4. Abbreviation for unigenes: beta-mannosidase (β-MAN), maltase A1 (MAL), glutathione-S-transferase 1 (GST-1) phospholipase D (PLD), trehalase (TRE), angiotensin-converting enzyme (ACE), regucalcin (RGN), reticulocalbin-2 (RCN), calumenin (CALU). Tissues: salivary glands (SG); alimentary canals (AC); whole body of apterous adult (WB); whole body minus salivary glands of apterous adult (-SG). β- actin and NADH were used as the internal reference genes. Standard error (SE) is represented by the error bar. Different small letters above each bar indicate significant differences (P < 0.05).