Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 21;7:15897. doi: 10.1038/s41598-017-15607-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

IGF-1 enhanced the combination of β-catenin and PKM2. (A) Immunoprecipitation (Co-IP) was performed on 293 T cells transfected with β-catenin and PKM2. After transfection for 48 hours, cells were subjected to Co-IP analysis using the indicated β-catenin and PKM2 antibodies for IP and blotting, with 10% of input proteins as indicated. (B) MCF7 cells were infected with lentivirus carrying sh-NC or sh-PKM2 plasmid and selected using puromycin. Then the cells were treated with IGF-1 for 24 hous after depletion of PKM2. Protein expression levels of PKM2 and β-catenin were analyzed by Immunoblotting assay (left), and the expression level of β-catenin was normalized to β-actin (right). The results were from three different replicates. (C) The stable PKM2-overexpressing MCF7 cells were transfected with TOP-FLASH/ FOP-FLASH plasmids and subsequently treated with IGF-1 for 24 hours. Luciferase activities were determined by the dual-luciferase reporter assay system. *Indicates p < 0.05 when compared to negative control (NC, without PKM2 overexpression) without IGF-1 treatment. ^#indicates p < 0.05 when compared to NC with IGF-1 treatment. (D) MCF7 cells stably expressed miR-NC or miR-152 were treated with 100 nM IGF-1 for 0, 8 and 24 hours. The levels of indicated proteins were analyzed by Immunoblotting. (E) MiR-152 expression levels in MCF7 and MDA-MB-231 cells overexpressing sh-RNA negative control or sh-PKM2 or sh-β-catenin in combination with IGF-1 treatment for 24 hours were analyzed using qRT-PCR. *Indicates p < 0.05 when compared to negative control without any treatment; ^#indicates p < 0.05 when compared to IGF-1 treatment group.