Table 3.
Single channel properties of GABAA receptors containing the β3 mutations L170R, A305V, and T288N.
| α1β3γ2L | α1β3L170Rγ2 | α1β3A305Vγ2 | α1β3T288Nγ2 | ||
|---|---|---|---|---|---|
| Number of openings | 15128 (n = 7) | 42918 (n = 11) | 13853 (n = 9) | 23189 (n = 6) | 10177 (n = 6) |
| i, pA | 2.03 ± 0.37 | 1.81 ± 0.20 | 1.52 ± 0.08*** | 1.59 ± 0.04** | 0.93 ± 0.198 |
| Open probability | 0.78 ± 0.16 | 0.28 ± 0.14*** | 0.42 ± 0.34* | 0.52 ± 0.26 | 0.42 ± 0.22* |
| Mean open time, ms | 12.8 ± 3.17 | 4.59 ± 0.56**** | 17.0 ± 3.10** | 6.74 ± 1.43*** | 11.2 ± 4.39 |
| O1, ms (%) | 2.21 (72) | 3.68 (82) | 4.61 (41) | 3.71 (83) | 1.96 (55) |
| O2, ms (%) | 12.7 (13) | 6.82 (15) | 21.8 (58) | 13.7 (13) | 11.4 (37) |
| O3, ms (%) | 59.2 (15) | 14.3 (3) | 13.7 (13) | 44.9 (4) | 67.6 (8) |
| Burst duration, ms | 29.4 ± 10.5 | 10.5 ± 1.47**** | 42.2 ± 10.0** | 25.3 ± 4.90 | 17.9 ± 5.89* |
| OB1, ms (%) | 3.37 (80) | 3.53 (49) | 4.35 (46) | 10.3 (72) | 2.57 (69) |
| OB2, ms (%) | 129 (20) | 16.8 (51) | 73.1 (54) | 60.1 (28) | 49.0 (31) |
Values represent mean ± S.D. *, **, *** and ****indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test) statistically different from α1β3γ2, respectively.