FIG 2 .

EtpE-C prevents latex bead-induced ROS generation by BMDMs from WT, but not DNase X^−/−, mice. BMDMs from WT (A, B) and DNase X^−/− (C, D) mice were preincubated with luminol in HBSSd for 15 min and then incubated with 40 ng of EtpE-C- or EtpE-N-coated or uncoated beads (~5 × 10⁶) or HBSSd (control [CTL]), and ROS generation was recorded as the relative chemiluminescence of oxidized luminol (A, C). The area under the curve was measured over 30 min after bead addition and is shown relative to ROS generation with uncoated beads, which was considered 100% (B, D). Results are presented as the mean ± the standard deviation of at least three independent experiments and were compared with a Student t test; *, P < 0.05.