FIG 3 .

EtpE-C prevents latex bead-induced ROS generation by BMDMs from WT mice in a dose-dependent manner. BMDMs from WT (A, B) and DNase X^−/− (C, D) mice were preincubated with luminol in HBSSd for 15 min and then incubated with beads coated with 40, 20, or 0 ng of EtpE-C (~5 × 10⁶) or with HBSSd (control [CTL]), and ROS generation was recorded as the relative chemiluminescence of oxidized luminol (A, C). The area under the curve was measured over 60 min after bead addition and is shown relative to ROS generation with uncoated (0 ng of EtpE-C) beads, which was considered 100% (B, D). Results are presented as the mean ± the standard deviation of at least three independent experiments. The coefficient of correlation (r value) between the relative levels of ROS and the amounts of EtpE-C is 0.999 (P < 0.05).