FIG 4 .

EtpE-C-coated beads block PMA-induced ROS generation by BMDMs from WT mice but not by BMDMs from DNase X^−/− mice. BMDMs from WT (A, B) and DNase X^−/− (C, D) mice were preincubated with luminol in HBSSd for 15 min and then incubated with beads coated with 40 ng of EtpE-C or EtpE-N, uncoated beads (~5 × 10⁶), or with HBSSd (control [CTL]) at 37°C for 30 min. ROS generation was induced with PMA, recorded (A, C), and analyzed (B, D), and the results are presented as in Fig. 1; UN, unstimulated BMDMs in HBSSd without PMA addition. *, P < 0.05.