FIG 8 .

EtpE-C-coated beads do not induce reduction of p22^phox in BMDMs from WT or DNase X^−/− (KO) mice, and human monocytes. BMDMs from WT and DNase X^−/− mice (A, C, D) or human peripheral blood monocytes (B, E) were incubated with EtpE-C- or EtpE-N-coated beads at 37°C for 2 h. Whole-cell lysates were prepared and subjected to Western blotting with antibodies against NOX2, p22^phox, and actin. (C to E) Relative amounts of p22^phox and NOX2 were calculated by normalizing the band intensities with actin, and the ratio observed in the control group (incubated with EtpE-N-coated beads) was arbitrarily set to 100. Results are presented as the mean ± the standard deviation of at least three independent experiments.