FIG 6 .

SARS-CoV nsp3 and nsp4 also suffice to induce DMV formation. (A and B) 293T cells were cotransfected with plasmids encoding SARS-CoV nsp3 and nsp4 and fixed for ET analysis 24 h posttransfection. Two virtual slices (8.5 nm) from reconstructed tomograms (available as Movies S3 and S4, respectively) are shown. Red arrows indicate zippered ER, and red asterisks indicate all the DMVs in this virtual slice that were fully reconstructed in the tomogram. (C and D) 293T cells were cotransfected with plasmids encoding MERS-CoV nsp3 and nsp4 and fixed for conventional EM analysis 24 h posttransfection. DMVs are indicated with red asterisks, and red arrows point at zippered ER. Bars, 250 nm.