FIG 6 .
Subcellular fractionation of phage-treated MDCK and A549 cells. (A) Fractionation of MDCK cells treated with T4 phages (lysate) for 18 h. Cells were washed and lysed using the lysosome enrichment kit, and the total number of phages in cells (Total Cell Lysate) was determined. Total cell lysates were fractionated, and 10 cellular fractions were collected and split for either phage quantification by bacterial plating or Western blot analysis of Golgi apparatus and endoplasmic reticulum (ER). (B) Fractionation of A549 cells treated with T4 phages for 18 h. Scatter plots show medians; error bars represent 95% confidence intervals. Bar plot shows mean; error bars show standard deviations.