Figure 5.

Role of sAC in intracellular pH regulation in response to external acidification. External acidification was achieved by lowering seawater pH from 8.1 to 7.4 by adding HCl, in the dark (indicated by shaded boxes). (a) Coral cells without Symbiodinium (−Sym), and (b) coral cells with Symbiodinium (+Sym). Asterisks denote statistically significant differences between selected time points (50–59 cells from four different corals per time point, N = 4; RM-ANOVA; Bonferroni's multiple comparison test). Error bars indicate s.e.m.; where not visible they fall within the symbol of the data point.