Table 2.
Demographic analyses of nSSR data of S. zenkeri and G. suaveolens implemented with DIYABC and BOTTLENECK (figure 2). (a) Genetic cluster divergence, divergence of intraspecific genetic clusters: Pleistocene divergence (less than 2 Myr BP), Pliocene divergence (2–4 Myr BP), and Mio/Pliocene divergence (4–6 Myr BP). (b) Bottleneck, heterozygosity-excess tests (probability of constant population size) of each nSSR genetic cluster using a Wilcoxon's signed-rank test. The magnitude of deviation was estimated with the parameter T2 = (Hobserved−Hexpected)/s.d. under the TPM and SMM is indicated in parentheses. (c) Broad time models, for each intraspecific genetic cluster four demographic models were tested between the beginning of the PGM and the present (PGM, 190 kyr–0 yr BP): constant population size, decline, expansion, and bottleneck followed by expansion; (d) Narrow time models, constant population size, bottleneck during the PGM (190–120 kyr BP), and/or subsequent expansion; bottleneck during the Last Glacial Maximum (LGM, 70–10 kyr BP) and/or subsequent expansion; and bottleneck during the climatic pejoration of the recent Holocene (4–2 kyr BP) and/or subsequent expansion (less than 2 kyr BP). Posterior probability of the most likely scenarios is highlighted in bold. BF against the alternative scenarios in parentheses. Conf., confidence in scenario choice under the most likely scenario; abs (absolute), the probability of the least likely scenario is 0; PGM, Penultimate Glacial Maximum; LGM, Ultimate Glacial Maximum.
| (a) Genetic cluster divergence |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| less than 2 Myr BP | 2–4 Myr BP | 4–6 Myr BP | conf. | |||||||||
| Scorodophloeus zenkeri | 0.97 | 0.03 (32.9) | 0 (abs) | 0.83 | ||||||||
| Greenwayodendron suaveolens | 0.88 | 0.1 (8.4) | 0.02 (45.4) | 0.81 | ||||||||
| (b) bottleneck |
(c) broad time models |
(d) narrow time models |
||||||||||
| TP model | SM model | constant | decline | expansion | bottleneck | conf. | constant | PGM | LGM | Holocene | conf. | |
| Scorodophloeus zenkeri Northwest | 0.02 (−2.84) | 0 (−4.8) | 0 (571.9) | 0 (abs) | 0.43 (1.3) | 0.57 | 0.51 | 0.01 (116.04) | 0.99 | 0 (205.5) | 0 (2465.8) | 0.66 |
| Scorodophloeus zenkeri West | 0.23 (−1.17) | 0.01 (−3.66) | 0.29 (2.4) | 0 (2264) | 0.03 (20.7) | 0.68 | 0.46 | 0.07 (13.92) | 0.92 | 0.01 (74) | 0 (203.8) | 0.81 |
| Scorodophloeus zenkeri Southwest | 0 (−5.43) | 0 (−14.4) | 0 (1644.5) | 0 (abs) | 0.66 | 0.34 (1.9) | 0.9 | 0 (8211) | 0.82 | 0.18 (4.59) | 0 (abs) | 0.78 |
| Scorodophloeus zenkeri East | 0.38 (−1.81) | 0 (−7.33) | 0.25 (1.7) | 0 (4261) | 0.33 (1.3) | 0.43 | 0.46 | 0.14 (4.37) | 0.59 | 0.27 (2.2) | 0 (179.6) | 0.7 |
| Greenwayodendron suaveolens Northwest | 0.02 (−12.2) | 0.02 (−26.19) | 0.01 (108.9) | 0 (abs) | 0 (abs) | 0.99 | 0.44 | 0 (1110.11) | 1 | 0 (abs) | 0 (abs) | 0.7 |
| Greenwayodendron suaveolens Southwest | 0.02 (−5.41) | 0.01 (−11.33) | 0.08 (10) | 0 (abs) | 0.79 | 0.13 (6) | 0.89 | 0.07 (12.57) | 0.93 | 0 (abs) | 0 (abs) | 0.78 |
| Greenwayodendron suaveolens Northeast | 0.03 (−2.35) | 0 (−9.23) | 0.1 (7.4) | 0 (abs) | 0.73 | 0.17 (4.4) | 0.89 | 0.01 (78.37) | 0.99 | 0 (abs) | 0 (abs) | 0.75 |
| Greenwayodendron suaveolens Southeast | 0.02 (−8.72) | 0 (−18.24) | 0.01 (85.7) | 0 (abs) | 0.32 (2.1) | 0.67 | 0.41 | 0.01 (157.7) | 0.99 | 0 (3311.67) | 0 (abs) | 0.67 |